Quercetin inhibits airway allergic inflammation through inhibiting the expression of NF-κB activator 1
نویسندگان
چکیده
Objectives: Quercetin is a major bioflavonoid found in plants and used as antioxidant supplements on the market. Airway allergic inflammation is very common in asthma patients, which characterized by excessive eosinophils infiltration, lgE production and enhanced airway resistance due to Th-2 dominance. The present study was designed to investigate the anti-allergic potential of Quercetin as well as the underlying mechanism involved. Methods: An OVA-sensitized AHR animal model was established by inhalation of 1% OVA for consecutive 14 days after an initial injection of 10% OVA intraperitoneally. Then, the airway resistance was assayed by Buxco Resistance and Compliance (RC) system; cell enumeration was assayed by direct microscopic observation; IFN-γ, IL-4 and IL-17 secretion in BALF and lgE production in serum were assayed by ELISA. Real-time PCR and immunohistochemistry were used to test the expressions of NF-κB activator 1 (Act1) which promotes allergic inflammation. Results: The results showed that Quercetin (50 mg/kg) decreased airway resistance, the number of total cells and eosinophils, the secretion of IL-4 and IL-17 and OVA-specific IgE induced by OVA. Moreover, Quercetin (50 mg/kg) also significantly decreased the expression of Act-1 induced by OVA. And the increase of Act-1 mRNA expression was obviously positively associated with the increased eosinophils infiltration (P=0.0013), secretion of IL-4 (P=0.0006) and IL-17 (P=0.0034) and production of lgE antibody (P=0.0074). Conclusions: Our data suggest that Quercetin effectively inhibits allergic inflammation including reductions of airway responsiveness, eosinophil infiltration, IL-4 and IL-17 secretion and lgE production by inhibiting the expression of Act1.
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